Study on the metabolic potential of six ancient bacterial lineages in deep-sea sediments using MAGs macrogene assembly method

Tech 2023-05-30 09:35:43 Source: Network

//CR(MAGs)6(Lokiarchaeota, Thorarchaeota, Heimdallarchaeota, Bathyarcheota, ThermoplasmatalesHadesarchaea)31magLokiarchaeotaBathyarchaeotamagCRLokiarchaeotaLokiarchaeotaWood-LjungdahlcomarchaeotaarchaeotaCR31(MAGs)6(Lokiarchaeota, Thorarchaeota, Heimdallarchaeota, Bathyarcheota, ThermoplasmatalesHadesarchaea)LokiarchaeotaBathyarchaeotaMAGsLokiarchaeotaDNAIQ-Tree16(L2L3L4L5L6L14L15L16L18L22L24S3S8S10S17S19)(VT + F + R10) 1000HMMsearchHMMMAGsBlastKoalKEGGUSEARCH-ublastMEROPSbinKEGG,CR Lokiarchaeota(rG)pH81mmCRU1378U1379CR Lokiarchaeota:G0rRCHNOSZ(4C)Qr16S rRNACR5,12616S rRNA(3125%)archaeotaLokiarchaeota531mag11(60%5%)magNCBI (nr)MAG(60-75%)CR_1215%Heimdallarchaeota16MAGs6Lokiarchaeota, Thorarchaeota, Heimdallarchaeota, Bathyarcheota, ThermoplasmatalesHadesarchaeaCRMAGs(ESPs)espCRHeimdallarchaeotaLokiarchaeota MAGs (CR_06, CR_11)ESPsCRarchaeota, HadesarchaeotaThermoplasmata MAGsespMCR(mcrABCDG)CRMCRHMMmag(AssA)(BssA)C-CLokiarchaeota CR_06Lokiarchaeota CR_06BCRBBzd4(BzdONPQ)BCRiCR_06BCR(LokiarchaeotaBathyarchaeotaArchaeoglobus)BCRPQATP/ATP[4Fe-4S]a(ON)aaCR_06BCR4(CR_06-contig-100_3495)CR_06 MAG(= 1110)> 200xCR_ Lokiarchaeota (CR_06)CRAsgardAsgard MAGs(CR_06, 07, 08, 1112)CO2H2OrG '(rG ' = 196.3 [kJ/mol])CR(86%)Lokiarchaetoa():(a)CO2H2O(rG ' =1206

CR(MAGs)

6(Lokiarchaeota, Thorarchaeota, Heimdallarchaeota, Bathyarcheota, ThermoplasmatalesHadesarchaea)31magLokiarchaeotaBathyarchaeotamag

LokiarchaeotaLokiarchaeota

Wood-Ljungdahlcom

archaeota

31(MAGs)6(Lokiarchaeota, Thorarchaeota, Heimdallarchaeota, Bathyarcheota, ThermoplasmatalesHadesarchaea)

LokiarchaeotaBathyarchaeotaMAGsLokiarchaeota

DNA

IQ-Tree16(L2L3L4L5L6L14L15L16L18L22L24S3S8S10S17S19)

(VT + F + R10) 1000

HMMsearchHMMMAGs

BlastKoalKEGG

USEARCH-ublastMEROPSbin

KEGG,

CR Lokiarchaeota(rG)pH81mm

CRU1378U1379CR Lokiarchaeota

G0rRCHNOSZ(4C)

16S rRNA

CR5

,12616S rRNA

(3125%)archaeotaLokiarchaeota

531mag11(60%5%)mag

NCBI (nr)

MAG(60-75%)CR_1215%Heimdallarchaeota

16MAGs6Lokiarchaeota, Thorarchaeota, Heimdallarchaeota, Bathyarcheota, ThermoplasmatalesHadesarchaea

CRMAGs(ESPs)esp

CRHeimdallarchaeotaLokiarchaeota MAGs (CR_06, CR_11)ESPs

CRarchaeota, HadesarchaeotaThermoplasmata MAGsesp

MCR(mcrABCDG)CRMCR

HMMmag

(AssA)(BssA)C-C

Lokiarchaeota CR_06Lokiarchaeota CR_06BCRBBzd4(BzdONPQ)

BCRiCR_06BCR(LokiarchaeotaBathyarchaeotaArchaeoglobus)BCR

PQATP/ATP[4Fe-4S]

a(ON)aa

CR_06BCR4(CR_06-contig-100_3495)

CR_06 MAG(= 1110)> 200x

CR_ Lokiarchaeota (CR_06)CRAsgard

Asgard MAGs(CR_06, 07, 08, 1112)CO2H2O

rG '(rG ' = 196.3 [kJ/mol])CR

(86%)

Lokiarchaetoa

():

(a)CO2H2O(rG ' =1206.3 [kJ/mol]);

(b)CO2H2O(rG ' =373.6 [kJ/mol]);

(c)CO2H2O(rG ' = - 119.9 [kJ/mol])

Lokiarchaeota

[NiFe]3b3dNADH+NADPH+H2Asgard

(IV)()CR_06

CR_14MCR(mcrABCDG)

CR_144()(TMAO/DMSO)n -(TMAO)

corcoroidCoB - CoM/F420(hdrABCDmvhADG)CR_14M (CoM)B (CoB)CoM- sh

s -(mtrA- h)mtrACoM5,6,7,8-(H4MPT)CODH/ACSaWood-Ljungdahl

CR_14MAG

archaeota CR_14archaeota

a(AcsB)CR_14 AcsBAsgardChloroflexiAltiarchaeales

AltiarchaealesAcsB

ChloroflexiACSarchaeotaAsgardAltiarchaealesACS

ACSarchaeotaACS(CoM, H4MPT)aarchaeota

CRMAGsESPESPs

archaeotaLokiarchaeotamag

(Aminicenantes, TA06, Aerophobetes, Atribacteria, HelarchaeotaBathyarchaeota)

CR_Asgard/CR_Lokiarchaeota/atpBCR(Bzd)

Lokiarchaeota()

531mag(Lokiarchaeota, Thorarchaeota, Heimdallarchaeota, Bathyarcheota, ThermoplasmatalesHadesarchaea)

11mag(>60%<10%)

90%archaeotaAsgardMAGMAG

()

Lokiarchaeota

Lokiarchaeota

archaeota

mag

CRMAGs

()Wood-Ljungdahl

[1]

[2]

[3]

[4]

[5]

[6]

[7]Muhammad Zohaib Nawazsmall RNA

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Tag: Study on the metabolic potential of six ancient bacterial