Bioengineering silkworms with butterfly cytotoxic modified silk glands can produce sericin protein cocoons
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||(PSGs)pierisin-1A (P1A)P1AP1APSGP1APierisin-175%P1AADP[32P]NADADPDNAHPLCpierisin-1P1ADNA ADP5%P < 0.001P1ANDNA ADPP1A269B
![](/attached/image1/2023/06/01/899eba757cf88e6633673ab8017800ac.gif)
![](/attached/image1/2023/06/01/490bc427e51acf5adfb71aac340f8cb1.jpeg)
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(PSGs)pierisin-1A (P1A)P1A
![](/attached/image1/2023/06/01/e65472c9bcc26ef6257103a162970236.png)
P1APSG
P1APierisin-175%P1AADP[32P]NADADPDNAHPLCpierisin-1P1ADNA ADP5%P < 0.001
P1ANDNA ADPP1A269B. moriBM-NSpodoptera frugiperdaSf21
![](/attached/image1/2023/06/01/eb2df64a22ac1686b8ae0c3ba4731e7e.png)
P1A269P1ABM-N
P1A269w1-pndP1A269/P1A269FibHP1A269PSGP1A269PSGP1APSGC
![](/attached/image1/2023/06/01/e4f4c4d3dec838137374a0c5e30cf9b7.png)
PCRDNA16SDS/PAGEP1A2696PSGP1A269P1A269Sf21BM-N
P1A2694367RT-PCRP1A269mRNA36
![](/attached/image1/2023/06/01/a259139b3616ca5728bef7dfb3aea7fb.png)
w1-pndP1A269/P1A269PSGw1-pnd+/+PSGw1-pndP1A269/P1A26967
3PSGFibH4
![](/attached/image1/2023/06/01/3a95814e4a02450a05a99f681d343339.png)
P1A269436P1A269PSG
w1-pndP1A269/P1A269
w1-pndP1A269/P1A269w1-pnd+/+79%P < 0.00170%[FibHFibL]25%
![](/attached/image1/2023/06/01/909b1cd6424086b665f4b27d40db54e3.jpeg)
PSGw1-pndP1A269/P1A269
5-20%SDS/PAGEFibLFibLFibHw1-pnd+/+w1-pndP1A269/P1A2691-4
![](/attached/image1/2023/06/01/e0ad8b3345f645f5f30e2bf5944d9e16.jpeg)
RT-PCRw1-pndP1A269/P1A269PSGFibHFibLmRNA5677w1-pndP1A269/P1A269FibHFibL mRNAw1-pnd+/+FibHFibL mRNA3.1%0.9%P < 0.001
w1-pnd+/+A3w1-pndP1A269/P1A269PSGmRNAP1A269PSGFibHFibL
![](/attached/image1/2023/06/01/2990039517cadd8fe6986e90424e6fef.png)
w1-pndP1A269/P1A269w1-pnd+/+1.51.3P < 0.001
GlyThrSerTyr
![](/attached/image1/2023/06/01/f42df9b45453a7f31adcfded48175acd.jpeg)
w1-pndP1A269/P1A269
Takasu-2
![](/attached/image1/2023/06/01/3e7d0947f8e45d4b7e8e3083d418a7c9.jpeg)
P1A269
KWP1A269/P1A269Kinshu x Showaw1-pndP1A269/P1A269EGFPw1-pnd+/+
![](/attached/image1/2023/06/01/fc740fdef8eefe92ab3cbd4526834af1.jpeg)
KWP1A269/P1A269FibLFibHSDS/PAGEFibL
KWP1A269/P1A269w1-pndP1A269/P1A2691.7P < 0.001w1-pndP1A269/P1A269
shigen.nig.ac.jp/silkwormbase/top.jsp
pHSDS/PAGESDS/PAGE6M LiBrw1-pndP1A269/P1A269
![](/attached/image1/2023/06/01/c6501c4985ba4b28df082ad00473d3bd.jpeg)
10%1
96%42-34
![](/attached/image1/2023/06/01/2d6cb4395fd9643a6559a987514ddae1.jpeg)
ESEB5rhLIFLIF
![](/attached/image1/2023/06/01/f98ddad1789fb0bb21de84c1858513dd.png)
LIFEB5ALPEB5blasticidinblasticidin
EB5ALPALP
![](/attached/image1/2023/06/01/e0a98795edebe609b29571ebfdbcc5d6.jpeg)
LIFEB5rhLIFEB5ALPALPrhLIF1418P0.0053BP0.05LIFEB5
w1-pndP1A269/P1A269PSGP1ADNA ADPP1A269P1APierisin-1
![](/attached/image1/2023/06/01/e5d3814b6539bf9c0beb6c6726b3f158.png)
P1A269BM-NP1A269BM-N
BM-NP1A269DNA
![](/attached/image1/2023/06/01/5fedfb444e7f90fa69bc20e84af97aff.jpeg)
P1A269PSGFibHFibL mRNAPSGP1A269FibHFibL
A3 mRNAP1A269P1A269P1A269P1A269PSG
![](/attached/image1/2023/06/01/93a703ecbb73809b8710833c64e37790.png)
P1A269A3 mRNAP1A269PSGrRNAsP1A26918S rRNA
Pierisin-1Pierisin-1
![](/attached/image1/2023/06/01/3c63fa06fee46fe64813ff2ef258c11a.png)
P1AP1A N
P1ANP1A
![](/attached/image1/2023/06/01/c5047eab4fe76cc3e3a9883ffb24396c.jpeg)
P1AP1APSGDNA ADP
BM-NP1A269Sf21PSGDNA ADPP1A269P1A269P1A
![](/attached/image1/2023/06/01/b6229b3e61d2f03a7ab3e34cca1e8c16.png)
w1-pndP1A269/P1A269Bcl-2XPSG
T7 RNAPSGFibLFibHFibHFibHFibL
![](/attached/image1/2023/06/01/7833d2e4d4fff87f42945d2c4f157516.jpeg)
NdPSGFibHFibL
P1A
[1] Mineralization and Biocompatibility of Antheraea pernyi (A. pernyi) Silk Sericin Film for Potential Bone Tissue Engineering. Feng Liu;;Ali Goodarzi;;Haifeng Wang;;Joanna Stasiak;;Jianbo Sun;;Yu Zhou;;Mingying Yang;;Namita Mandal;;Yajun Shuai;;Guanshan Zhou;;Sijia Min;;Liangjun Zhu.Bio-Medical Materials and Engineering,2014
[2] Preparation and Characterization of Silk Sericin/Glycerol/Graphene Oxide Nanocomposite Film. Haesung Yun;;Moo Kon Kim;;Hyo Won Kwak;;Jeong Yun Lee;;Min Hwa Kim;;Eui Hwa Kim;;Ki Hoon Lee.Fibers and Polymers,2013
[3] Preliminary Characterization of Genipin-Cross-Linked Silk Sericin/Poly(vinyl alcohol) Films as Two-Dimensional Wound Dressings for the Healing of Superficial Wounds. Tippawan Siritientong;;Juthamas Ratanavaraporn;;Teerapol Srichana;;Pornanong Aramwit;;Joshua R. Mauney.BioMed Research International,2013
[4] Potential of 2D crosslinked sericin membranes with improved biostability for skin tissue engineering. Sunita Nayak;;Sarmistha Talukdar;;Subhas C. Kundu.Cell and Tissue Research,2012
[5] Evaluation of sericin/collagen membranes as prospective wound dressing biomaterial. Omer Akturk;;Aysen Tezcaner;;Hasan Bilgili;;M. Salih Deveci;;M. Rusen Gecit;;Dilek Keskin.Journal of Bioscience and Bioengineering,2011
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